Over the past two decades, extensive collections of zebrafish lines carrying mutations in genes involved in early embryonic development have been generated—some of these mutations in genes important for eye formation and associated with eye malformations in humans. Besides, many transgenic lines have been generated at Creative Biogene to enable the close monitoring of organogenesis and the manipulation of gene activity in a tissue-directed way. We mainly use the three approaches to abrogate gene function in zebrafish: stable lines carrying mutations (mutants); morpholino-based knock-down of gene function; and use of CRISPR/Cas9 gene editing to disrupt gene function in injected embryos. Many human pathological conditions from Alzheimer's disease to metabolic syndrome have been successfully modelled in zebrafish. Zebrafish Ocular Disease Models
